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KMID : 0350519950480030887
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Journal of Catholic Medical College
1995 Volume.48 No. 3 p.887 ~ p.897
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Loss of Heterozygosity on Chromosome 13q in Head and Neck Squamous Ceell Carcinoma using Microsatellite Markers
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Abstract
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Loss of heterozygosity on chromosome 13q is a common feature in cancer tissues of the head and neck, parathyroid, ovary, esophagus, kidney, lung, bladder and breast. This high rate of allele loss suggests that one or more tumor suppressor genes
on
chromosome 12q are commonly involved in many tumor types. Recent studies using microsatellite markers have shown the multiple chromosome loss on 3p, 5q, 13q and 17p in head and neck squamous cell carcinoma. Especially chromosome 13q contained Rb
gene
locus is so interesting because it is possible that there are candidate genes other than Rb gene according to the studies of loss of heterozygosity on chromosome 13q, To be certain of the possibility of new tumor suppressor gene near to Rb gene,
we
performed a detailed examination of the squamous cell carcinoma of head and neck in eighteen patients for loss of heterozygosity or microsatellite instability at loci D13S115, D13S118, D13S119, D13S128, D13S133, D13S135, D13S170 and D13S64 of
chromosome
13q. Allelic alterations of tumor tissues at all of 8 loci were analyzed by electrophoretic patterns of polymerase chain reaction products of microsatellite markers on sequencing gel.
1. Thirteen out of eighteen patients (72.2%) revealed loss of heterozygosity or instability at one or more loci at the checked loci of chromosome 13q.
2. Especially loss of heterozygosity or microsatellite instability were frequently involved at loci D13S118 (22.2%), D135133 (38.9%), D13S135 (33.3%) and D13S170 (33.3%)
These results implicate that another putative suppressor gene might exist at a distal portion of Rb gene locus on chromosome 13p.
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